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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0508.xml
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|a (DE-627)NLM152355383
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|a (NLM)15568863
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Vaidya, Ashish A
|e verfasserin
|4 aut
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|a DNA attachment chemistry at the flexible silicone elastomer surface
|b toward disposable microarrays
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 30.05.2006
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|a Date Revised 21.11.2008
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|a published: Print
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|a Citation Status MEDLINE
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|a This paper describes the preparation and surface characterization of maleimide-activated silicone elastomer (PDMS(MCC)) followed by covalent functionalization using thiol-terminated DNA sequences (primary oligo). The stability of this attachment chemistry was demonstrated by the retention of the primary oligo through the process of hybridization with a labeled complementary DNA sequence. In these studies, the hybridized labeled DNA oligomers were detected using confocal fluorescence microscopy. We have employed a vapor deposition technique in which a plasma-treated silicone elastomer (PDMS(OH)) was exposed to vapors of 3-(aminopropyl)triethoxysilane (APTS) under vacuum, to yield the amine-functionalized silicone elastomer (PDMS(NH)(2)). PDMS(NH)(2) was further coupled with a heterofunctional cross-linker, sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate to obtain PDMS(MCC). The surface functionalities of the elastomers were characterized using contact angle measurements and X-ray photoelectron spectroscopy. Surface-modified silicone elastomers appear to be promising substrates for use as substrates for disposable microarrays
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Silicone Elastomers
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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|a Norton, Michael L
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 25 vom: 07. Dez., Seite 11100-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:20
|g year:2004
|g number:25
|g day:07
|g month:12
|g pages:11100-7
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