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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n1221.xml
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|a (DE-627)NLM152036504
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|a (NLM)15535124
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Pacoda, Daniela
|e verfasserin
|4 aut
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|a Reactive oxygen species and nitric oxide affect cell wall metabolism in tobacco BY-2 cells
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 27.12.2004
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a The effects of hydrogen peroxide (H2O2), nitric oxide (NO), and a combination of both on the metabolism of cell wall polysaccharides were studied in tobacco (Nicotiana tabacum L.) cv Bright Yellow 2 (BY-2) suspension cultured cells in the presence of D-[U-14C]glucose or D-[U-14C]galactose as radioactive tracers. We found that the radiolabelling of newly synthesised total cell wall polysaccharides (pectins, hemicelluloses and alpha-cellulose), buffer-soluble polysaccharides, and membrane-associated polysaccharides decreased under the influence of exogenous systems generating H2O2 and NO. However, when the total amount of newly synthesised cell wall polysaccharides was calculated as a percentage of the total cellular radioactivity (ethanol-soluble pool plus the homogenate of ethanol-insoluble material), all treatments showed negligible effects in the presence of D-[U-14C]glucose or D-[U-14C]galactose as tracers. This occurred because the treatments generating H2O2, NO and H2O2 plus NO caused a marked decrease in the concentration of the ethanol-soluble pool as well as in the total radioactivity found in the homogenate of the ethanol-insoluble material. Most of the radioactivity taken up by the cells was evolved as 14CO2 during the respiratory processes. A qualitative and quantitative characterisation of the ethanol-soluble pool showed that radioactive UDP-sugars in BY-2 suspension cultured cells were differentially reduced by all treatments. Therefore, the decrease of the newly synthesised cell wall polysaccharides seems to be strictly dependent on the reduction of the UDP-sugars pool
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Polysaccharides
|2 NLM
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|a Reactive Oxygen Species
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|a Nitroprusside
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|a 169D1260KM
|2 NLM
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|a Nitric Oxide
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|a 31C4KY9ESH
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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|a Glucose Oxidase
|2 NLM
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|a EC 1.1.3.4
|2 NLM
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|a Glucose
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|a IY9XDZ35W2
|2 NLM
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1 |
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|a Montefusco, Anna
|e verfasserin
|4 aut
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1 |
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|a Piro, Gabriella
|e verfasserin
|4 aut
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| 700 |
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|a Dalessandro, Giuseppe
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 161(2004), 10 vom: 19. Okt., Seite 1143-56
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:161
|g year:2004
|g number:10
|g day:19
|g month:10
|g pages:1143-56
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 161
|j 2004
|e 10
|b 19
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|h 1143-56
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