|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM151878773 |
| 003 |
DE-627 |
| 005 |
20231223060855.0 |
| 007 |
tu |
| 008 |
231223s2004 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0506.xml
|
| 035 |
|
|
|a (DE-627)NLM151878773
|
| 035 |
|
|
|a (NLM)15518519
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ottaviani, M Francesca
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Interactions of dendrimers with selected amino acids and proteins studied by continuous wave EPR and Fourier transform EPR
|
| 264 |
|
1 |
|c 2004
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 10.04.2006
|
| 500 |
|
|
|a Date Revised 24.11.2016
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Interactions of polyamidoamine dendrimers, termed Gn, where n indicates the generation (=number of amidoamine layers), at different protonation levels with selected amino acids and proteins have been investigated by means of continuous wave electron paramagnetic resonance (cw-EPR) and pulsed-EPR (electron spin-echo = ESE) analyses. A low-generation dendrimer (G2) and a high-generation one (G6) were labeled with nitroxides for the EPR measurements. Gly, Glu, Arg, and Leu were selected as representative of neutral(zwitterionic)-polar, acidic, basic, and low-polar amino acids, respectively. The water-soluble proteins alpha-chymotrypsin and albumin were selected on the basis of a basic and an acidic isoelectric point, respectively. The cw-EPR spectra were analyzed by computing the line shapes to extract information about the dendrimer-biomolecule interactions. In general, dendrimers at a high protonation level interact stronger with amino acids than those at a low level of protonation. However, even for highly protonated dendrimers, a synergistic effect between hydrophilic and hydrophobic interactions promoted the formation of stable Gn-amino acid adducts, as demonstrated by the enhanced interactions with Leu. As expected from acid-base interactions, stable adducts were formed between Arg and highly protonated dendrimers and between Glu and low level protonated dendrimers. The relatively strong dendrimer interactions with the protein chymotrypsin and the poor interactions of dendrimers with albumin demonstrated that the protonated amino groups of the dendrimers are predominantly involved in the interactions with these proteins and indicated a significant role in the interactions with the dendrimers of the hydrophobic external residues of chymotrypsin. Computer-aided analysis of the ESE experiments was consistent with the cw-EPR results and supported the conclusion of a partial complexation of the nitroxides of the dendrimer with Leu and alpha-chymotrypsin
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Albumins
|2 NLM
|
| 650 |
|
7 |
|a Amino Acids
|2 NLM
|
| 650 |
|
7 |
|a Dendrimers
|2 NLM
|
| 650 |
|
7 |
|a PAMAM Starburst
|2 NLM
|
| 650 |
|
7 |
|a Polyamines
|2 NLM
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 650 |
|
7 |
|a Chymotrypsin
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.21.1
|2 NLM
|
| 650 |
|
7 |
|a alpha-chymotrypsin
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.21.1
|2 NLM
|
| 650 |
|
7 |
|a Leucine
|2 NLM
|
| 650 |
|
7 |
|a GMW67QNF9C
|2 NLM
|
| 700 |
1 |
|
|a Jockusch, Steffen
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Turro, Nicholas J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tomalia, Donald A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Barbon, Antonio
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 23 vom: 09. Nov., Seite 10238-45
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:20
|g year:2004
|g number:23
|g day:09
|g month:11
|g pages:10238-45
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 20
|j 2004
|e 23
|b 09
|c 11
|h 10238-45
|