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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed25n0506.xml
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|a (NLM)15517976
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Van Ert, Matthew N
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Mass spectrometry provides accurate characterization of two genetic marker types in Bacillus anthracis
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| 264 |
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|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 08.03.2005
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|a Date Revised 07.06.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a Epidemiological and forensic analyses of bioterrorism events involving Bacillus anthracis could be improved if both variable number tandem repeats (VNTRs) and single nucleotide polymorphisms (SNPs) could be combined on a single analysis platform. Here we present the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) to characterize 24 alleles from 6 VNTR loci and 11 alleles from 7 SNP loci in B. anthracis. The results obtained with ESI-FTICR-MS were consistent with independent results obtained from traditional approaches using electrophoretic detection of fluorescent products. However, ESI-FTICR-MS improves on the traditional approaches because it does not require fluorescent labeling of PCR products, minimizes post-PCR processing, obviates electrophoresis, and provides unambiguous base composition of both SNP and VNTR PCR products. In addition, ESI-FTICR-MS allows both marker types to be examined simultaneously and at a rate of approximately 1 sample per min. This technology represents a significant advance in our ability to rapidly characterize B. anthracis isolates using VNTR and SNP loci
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| 650 |
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4 |
|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Genetic Markers
|2 NLM
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| 700 |
1 |
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|a Hofstadler, Steven A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Yun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Busch, Joseph D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wagner, David M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Drader, Jared J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ecker, David J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hannis, James C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huynh, Lynn Y
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schupp, James M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Simonson, Tatum S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Keim, Paul
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 37(2004), 4 vom: 07. Okt., Seite 642-4, 646, 648 passim
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
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|g volume:37
|g year:2004
|g number:4
|g day:07
|g month:10
|g pages:642-4, 646, 648 passim
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|d 37
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|h 642-4, 646, 648 passim
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