Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals

Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals

In this paper, we describe a rapid and accurate real-time quantitative PCR-based system to determine transgene copy number in transgenic animals. We used the 2(-deltadeltaCt) method to analyze different transgenic lines without the requirement of a control sample previously determined by Southern bl...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 37(2004), 4 vom: 07. Okt., Seite 610-3
1. Verfasser: Ballester, Maria (VerfasserIn)
Weitere Verfasser: Castelló, Anna, Ibáñez, Elena, Sánchez, Armand, Folch, Josep M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't RNA, Messenger Taq Polymerase EC 2.7.7.-