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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0505.xml
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|a (DE-627)NLM15147902X
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|a (NLM)15475375
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Massonneau, Agnès
|e verfasserin
|4 aut
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| 245 |
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|a Maize cytokinin oxidase genes
|b differential expression and cloning of two new cDNAs
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| 264 |
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 03.02.2005
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|a Date Revised 15.11.2006
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Cytokinin oxidases (CKOs) play a major role in the regulation of hormone levels in plants by irreversibly degrading cytokinins. Two new cDNAs from maize (CKO2 and CKO3) were cloned and CKO activity of a recombinant CKO3 enzyme was demonstrated. CKO2 and CKO3 encode flavoproteins with 93% identity among each other compared with 45% identity with CKO1. The respective genes were mapped to BIN 3.05/06 and BIN 8.06 which belong to duplicated regions of the maize genome. For a better understanding of the role of CKO2 and CKO3 in maize development, their expression profiles were analysed in different organs and during kernel development via semi-quantitative RT-PCR. Different spatial and temporal expression patterns were observed for the two genes, as well as for CKO1 and two additional genes CKO4 and CKO5. CKO2 to CKO5 genes were mainly expressed in vegetative tissues, with unique expression patterns. CKO1 was most strongly expressed in the kernel. All five genes were expressed at early stages of kernel development, a period when a peak in cytokinin levels and a high cell division rate in the endosperm have been described. However, each gene had its own expression profile with a major difference concerning the onset of expression
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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| 650 |
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|a Oxidoreductases
|2 NLM
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| 650 |
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|a EC 1.-
|2 NLM
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| 650 |
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|a cytokinin oxidase
|2 NLM
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| 650 |
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|a EC 1.5.99.12
|2 NLM
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| 700 |
1 |
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|a Houba-Hérin, Nicole
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pethe, Claude
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Madzak, Catherine
|e verfasserin
|4 aut
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| 700 |
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|a Falque, Matthieu
|e verfasserin
|4 aut
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| 700 |
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|a Mercy, Mathieu
|e verfasserin
|4 aut
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| 700 |
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|a Kopecny, David
|e verfasserin
|4 aut
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| 700 |
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|a Majira, Amel
|e verfasserin
|4 aut
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| 700 |
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|a Rogowsky, Peter
|e verfasserin
|4 aut
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| 700 |
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|a Laloue, Michel
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 408 vom: 15. Dez., Seite 2549-57
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:55
|g year:2004
|g number:408
|g day:15
|g month:12
|g pages:2549-57
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|a AR
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|d 55
|j 2004
|e 408
|b 15
|c 12
|h 2549-57
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