Development of a universal gap repair vector for yeast-based screening of knockout rodents

Recently, we reported the production of the first knockout rats by combining N-ethyl-N-nitrosourea (ENU)-induced mutagenesis with a yeast-based truncation screening method. To make this new knockout technology more applicable for other laboratories and for high-throughput applications, we have devel...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 37(2004), 3 vom: 29. Sept., Seite 383-8
1. Verfasser: Chen, Kai-Shun (VerfasserIn)
Weitere Verfasser: Gould, Michael N
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S. Technical Report Adenomatous Polyposis Coli Protein BRCA1 Protein BRCA2 Protein DNA, Complementary