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231223s2004 xx ||||| 00| ||eng c |
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|a (NLM)15451471
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Yue-Dan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2:Ig fusion protein
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|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 19.11.2004
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|a Date Revised 07.12.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2+ SARS-recovered donors at different time points post disease. CD8+DimerX-S1203+ CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8+DimerX-S1203+ cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Epitopes, T-Lymphocyte
|2 NLM
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|a HLA-A2 Antigen
|2 NLM
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| 650 |
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|a Immunoglobulins
|2 NLM
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| 650 |
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|a Viral Fusion Proteins
|2 NLM
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| 650 |
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|a Iothalamic Acid
|2 NLM
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| 650 |
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|a 16CHD79MIX
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| 650 |
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|a 82115-62-6
|2 NLM
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| 700 |
1 |
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|a Chen, Wei Feng
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 113(2004), 2 vom: 29. Nov., Seite 151-4
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
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8 |
|g volume:113
|g year:2004
|g number:2
|g day:29
|g month:11
|g pages:151-4
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