Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis

Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis

We developed a method for obtaining viable buccal cells from mouthwash samples for use as a source of mRNA and protein. Immunofluorescent analysis showed that most cells were derived from nonkeratinized parabasal epithelia, with a minor proportion of proliferative cells. Gene expression was detected...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 37(2004), 2 vom: 01. Aug., Seite 262-4, 266-9
1. Verfasser: Michalczyk, Agnes (VerfasserIn)
Weitere Verfasser: Varigos, George, Smith, Lance, Ackland, M Leigh
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Journal Article Research Support, Non-U.S. Gov't Mouthwashes RNA, Messenger Keratins 68238-35-7