Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis
We developed a method for obtaining viable buccal cells from mouthwash samples for use as a source of mRNA and protein. Immunofluorescent analysis showed that most cells were derived from nonkeratinized parabasal epithelia, with a minor proportion of proliferative cells. Gene expression was detected...
| Publié dans: | BioTechniques. - 1991. - 37(2004), 2 vom: 01. Aug., Seite 262-4, 266-9 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2004
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| Accès à la collection: | BioTechniques |
| Sujets: | Evaluation Study Journal Article Research Support, Non-U.S. Gov't Mouthwashes RNA, Messenger Keratins 68238-35-7 |