Potential influence of the first PCR cycles in real-time comparative gene quantifications
There is an underlying assumption in real-time PCR that the amplification efficiency is equal from the first cycles until a signal can be detected. In this study, we evaluated this assumption by analyzing genes with known gene copy number using real-time PCR comparative gene quantifications. Listeri...
Veröffentlicht in: | BioTechniques. - 1993. - 37(2004), 2 vom: 01. Aug., Seite 246-8, 250-3 |
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Format: | Aufsatz |
Sprache: | English |
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2004
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't Bacterial Toxins DNA, Bacterial Heat-Shock Proteins Hemolysin Proteins RNA, Ribosomal, 23S hlyA protein, Listeria monocytogenes |