Potential influence of the first PCR cycles in real-time comparative gene quantifications

There is an underlying assumption in real-time PCR that the amplification efficiency is equal from the first cycles until a signal can be detected. In this study, we evaluated this assumption by analyzing genes with known gene copy number using real-time PCR comparative gene quantifications. Listeri...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 37(2004), 2 vom: 01. Aug., Seite 246-8, 250-3
1. Verfasser: Nogva, Hege Karin (VerfasserIn)
Weitere Verfasser: Rudi, Knut
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't Bacterial Toxins DNA, Bacterial Heat-Shock Proteins Hemolysin Proteins RNA, Ribosomal, 23S hlyA protein, Listeria monocytogenes R06ZRQ1YX9