Cloning and characterisation of a basic IAA oxidase associated with root induction in Vitis vinifera
Copyright 2004 Elsevier SAS
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 42(2004), 7-8 vom: 30. Juli, Seite 609-15 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA, Complementary Recombinant Proteins indoleacetic acid oxidase EC 1.11.- Peroxidases EC 1.11.1.- |
| Zusammenfassung: | Copyright 2004 Elsevier SAS Changes in apoplastic peroxidases during auxin-induced in vitro rooting of cultured grapevine (Vitis vinifera L. cv. Touriga) stems have been studied. The largest increase in peroxidase activity (EC 1.11.1.7) was associated with the early stages of root initiation and could be attributed to an increase in activity of an apoplastic 36 kDa cationic peroxidase (PxB2). Relative to other peroxidases, PxB2 demonstrated high indole-3-acetic acid (IAA) oxidase activity and apparently contributed the majority of potential IAA oxidase activity in rooting tissues. The distribution of this peroxidase in developing roots additionally associates it with early phases of growth restriction. PxB2 was purified from cell wall extracts prepared from the basal 1 cm of rooting stems. Microsequencing and subsequent cloning of its corresponding 3' truncated cDNA (encoding 255 amino acids of the mature protein) revealed it to have a typical class III peroxidase structure. The results suggest that this class III peroxidase with IAA oxidase activity is important for the control of IAA levels during root initiation and development |
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| Beschreibung: | Date Completed 20.12.2004 Date Revised 09.03.2022 published: Print Citation Status MEDLINE |
| ISSN: | 1873-2690 |