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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed25n0500.xml
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|a (DE-627)NLM149821808
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|a (NLM)15301511
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Yuan, Ruo
|e verfasserin
|4 aut
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|a Ultrasensitive potentiometric immunosensor based on SA and OCA techniques for immobilization of HBsAb with colloidal Au and polyvinyl butyral as matrixes
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 09.06.2006
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a A novel potentiometric immunosensor for detection of hepatitis B surface antigen (HBsAg) has been developed by means of self-assembly (SA) and opposite-charged adsorption (OCA) techniques to immobilize hepatitis B surface antibody (HBsAb) on a platinum electrode. A cleaned platinum electrode was first pretreated in the presence of 10% HNO3 and 2.5% K2CrO4 solution and held at -1.5 V (vs SCE) for 1 min to make it negatively charged and then immersed in a mixing solution containing hepatitis B surface antibody, colloidal gold (Au), and polyvinyl butyral (PVB). Finally, HBsAb was successfully immobilized onto the surface of the negatively charged platinum electrode modified nanosized gold and PVB sol-gel matrixes. The modified procedure was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The immobilized hepatitis B surface antibody exhibited direct electrochemical behavior toward hepatitis B surface antigen (HBsAg). The performance and factors influencing the performance of the resulting immunosensor were studied in detail. More than 95.7% of the results of the human serum samples obtained by this method were in agreement with those obtained by enzyme-linked immunosorbent assays (ELISAs). The resulting immunosensor exhibited fast potentiometric response (<3 min) to HBsAg. The detection limit of the immunosensor was 2.3 ng.mL(-1), and the linear range was from 8 to 1280 ng.mL(-1). Moreover, the studied immunosensor exhibited high sensitivity, good reproducibility, and long-term stability (>6 months)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Colloids
|2 NLM
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|a Hepatitis B Antibodies
|2 NLM
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|a Polyvinyls
|2 NLM
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|a Platinum
|2 NLM
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|a 49DFR088MY
|2 NLM
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|a Gold
|2 NLM
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| 650 |
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|a 7440-57-5
|2 NLM
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| 650 |
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|a polyvinylbutyral
|2 NLM
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| 650 |
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|a 9003-62-7
|2 NLM
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| 700 |
1 |
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|a Tang, Dianping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chai, Yaqin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhong, Xia
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Yan
|e verfasserin
|4 aut
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| 700 |
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|a Dai, Jianyuan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 20(2004), 17 vom: 17. Aug., Seite 7240-5
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:20
|g year:2004
|g number:17
|g day:17
|g month:08
|g pages:7240-5
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|a GBV_USEFLAG_A
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|a GBV_NLM
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|a AR
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|d 20
|j 2004
|e 17
|b 17
|c 08
|h 7240-5
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