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20231223052531.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0500.xml
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|a (DE-627)NLM149821786
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|a (NLM)15301509
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Dillmore, W Shannon
|e verfasserin
|4 aut
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| 245 |
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|a A photochemical method for patterning the immobilization of ligands and cells to self-assembled monolayers
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 09.06.2006
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|a Date Revised 30.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a This work describes a chemically well defined method for patterning ligands to self-assembled monolayers (SAMs) of alkanethiolates on gold. This method begins with monolayers presenting a nitroveratryloxycarbonyl (NVOC)-protected hydroquinone which is photochemically irradiated to reveal a hydroquinone group. The resulting hydroquinone is then oxidized to the corresponding benzoquinone, providing a site for the Diels-Alder mediated immobilization of ligands. The rate constant for the photochemical deprotection is 0.032 s(-1) (with an intensity of approximately 100 mW/cm(2) between 355 and 375 nm), corresponding to a half-life of 21 s. The hydroquinone is oxidized to the benzoquinone using either electrochemical or chemical oxidation and then functionalized by reaction with a cyclopentadiene-tagged ligand. Two methods for patterning the immobilization of ligands are described. In the first, the substrate is illuminated through a mask to generate a pattern of hydroquinone groups, which are elaborated with ligands. In the second method, an optical microscope fit with a programmable translational stage is used to write patterns of deprotection which are then again elaborated with ligands. This technique is characterized by the use of well-defined chemical reactions to control the regions and densities of ligand immobilization and will be important for a range of applications that require patterned ligands for biospecific interactions
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| 650 |
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|a Journal Article
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| 650 |
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4 |
|a Research Support, N.I.H., Extramural
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Benzoquinones
|2 NLM
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7 |
|a Cyclopentanes
|2 NLM
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|a Hydroquinones
|2 NLM
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7 |
|a Ligands
|2 NLM
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|a Membranes, Artificial
|2 NLM
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7 |
|a Oligopeptides
|2 NLM
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|a Sulfhydryl Compounds
|2 NLM
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|a Polyethylene Glycols
|2 NLM
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| 650 |
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|a 3WJQ0SDW1A
|2 NLM
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| 650 |
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|a Gold
|2 NLM
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| 650 |
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|a 7440-57-5
|2 NLM
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| 650 |
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|a arginyl-glycyl-aspartic acid
|2 NLM
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|a 78VO7F77PN
|2 NLM
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|a Fluorescein
|2 NLM
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|a TPY09G7XIR
|2 NLM
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| 700 |
1 |
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|a Yousaf, Muhammad N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mrksich, Milan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 17 vom: 17. Aug., Seite 7223-31
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:20
|g year:2004
|g number:17
|g day:17
|g month:08
|g pages:7223-31
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