"Lumen digestion" technique for isolation of aortic endothelial cells from heme oxygenase-1 knockout mice
Endothelial cell dysfunction plays a critical role in the pathogenesis of cardiovascular diseases. Gene targeted mutant, knockout. or transgenic mice are widely used in the laboratory investigation of these disorders. We describe a simple and reproducible "lumen digestion" technique to iso...
| Publié dans: | BioTechniques. - 1991. - 37(2004), 1 vom: 14. Juli, Seite 84-6, 88-9 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2004
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. DNA Primers Heme Oxygenase (Decyclizing) EC 1.14.14.18 Casp3 protein, mouse EC 3.4.22.- Caspase 3 Caspases |