Effect of NaCl and mannitol iso-osmotic stresses on proline and free polyamine levels in embryogenic Fraxinus angustifolia callus

With the aim to differentiate the ionic and osmotic components of salt stress, short and long-term changes in free polyamines and proline induced by iso-osmotic concentrations of NaCl (0.1 mol/L and 0.2 mol/L) and mannitol (0.2 mol/L and 0.4 mol/L) were determined in Fraxinus angustifolia callus. Th...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 161(2004), 6 vom: 15. Juni, Seite 701-8
1. Verfasser: Tonon, Giustino (VerfasserIn)
Weitere Verfasser: Kevers, Claire, Faivre-Rampant, Odile, Grazianil, Michele, Gaspar, Thomas
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Polyamines Spermine 2FZ7Y3VOQX Mannitol 3OWL53L36A Sodium Chloride 451W47IQ8X Proline 9DLQ4CIU6V mehr... Spermidine U87FK77H25 Putrescine V10TVZ52E4
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100 1 |a Tonon, Giustino  |e verfasserin  |4 aut 
245 1 0 |a Effect of NaCl and mannitol iso-osmotic stresses on proline and free polyamine levels in embryogenic Fraxinus angustifolia callus 
264 1 |c 2004 
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520 |a With the aim to differentiate the ionic and osmotic components of salt stress, short and long-term changes in free polyamines and proline induced by iso-osmotic concentrations of NaCl (0.1 mol/L and 0.2 mol/L) and mannitol (0.2 mol/L and 0.4 mol/L) were determined in Fraxinus angustifolia callus. The peculiarities of the short-term responses were: i) a very early (30 min) and temporary increase in Putrescine (Pu) and Spermine (Spm) as a consequence of salt treatment, and ii) a continuous accumulation of Spermidine (Spd) and Spm in response to mannitol. The changes of Proline (Pro) were quite limited both in the short and in the long term, and generally occurred later than Polyamine (PAs) changes took place, suggesting a regulatory mechanism of PAs metabolism on Pro biosynthesis. In the long-term, no drastic accumulations of Pro or PAs in response to NaCl and mannitol were observed, suggesting that their physiological role is unlikely to be that of osmo-compatible solutes in this plant system. The salt induced a higher callus growth inhibition effect than did mannitol and this inhibition was associated with the reduction of endogenous levels of PAs, especially Pu. However, while a diverging time course was observed under lethal salt concentration (0.2 mol/L NaCl), a high parallelism in the endogenous changes of Pro and Pu was observed under all non-lethal conditions (control--0.2 and 0.4 mol/L mannitol--0.1 mol/L NaCl). Therefore the synchronous changes of Pro and Pu can be considered as a physiological trait associated with cell survival. These results indicate a strong metabolic co-ordination between PAs and Pro pathways and suggest that the metabolic fluxes through these pathways start competing only when the stress level is high enough to be lethal for cells 
650 4 |a Journal Article 
650 7 |a Polyamines  |2 NLM 
650 7 |a Spermine  |2 NLM 
650 7 |a 2FZ7Y3VOQX  |2 NLM 
650 7 |a Mannitol  |2 NLM 
650 7 |a 3OWL53L36A  |2 NLM 
650 7 |a Sodium Chloride  |2 NLM 
650 7 |a 451W47IQ8X  |2 NLM 
650 7 |a Proline  |2 NLM 
650 7 |a 9DLQ4CIU6V  |2 NLM 
650 7 |a Spermidine  |2 NLM 
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650 7 |a Putrescine  |2 NLM 
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700 1 |a Kevers, Claire  |e verfasserin  |4 aut 
700 1 |a Faivre-Rampant, Odile  |e verfasserin  |4 aut 
700 1 |a Grazianil, Michele  |e verfasserin  |4 aut 
700 1 |a Gaspar, Thomas  |e verfasserin  |4 aut 
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