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DE-627 |
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20231223051504.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0498.xml
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|a (DE-627)NLM149327846
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|a (NLM)15248699
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Pantos, Alexandros
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Interactions of complementary PEGylated liposomes and characterization of the resulting aggregates
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| 264 |
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 07.06.2006
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|a Date Revised 30.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Copyright 2004 American Chemical Society
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|a The interaction of complementary liposomes bearing both recognizable and protective ligands at their external surface has been investigated. Aggregation of hydrogenated phosphatidyl choline/cholesterol (2:1 molar ratio) based liposomes was mediated by the molecular recognition of the complementary phosphate and guanidinium groups incorporated in separate unilamellar liposomes. The phosphate group was incorporated in the bilayer employing dihexadecyl phosphate, while the guanidinium moiety was introduced in the membrane through the incorporation of various guanidinium lipids. For the latter, anchoring ability and primarily introduction of a spacer group between their lipophilic part and the guanidinium group was found to affect the ability for molecular recognition. Also, poly(ethylene glycol) (PEG) introduced in both types of liposomes at various concentrations and up to 15% with respect to cholesterol modifies the interaction effectiveness and morphology of the obtained aggregates. Interaction of these complementary liposomes leads to large precipitating aggregates or fused liposomes, as shown by phase contrast microscopy and dynamic light scattering. Specifically, fusion of liposomes takes place under a nonleaking process involving lipid mixing, as demonstrated by calcein entrapment and resonance energy transfer experiments. Calorimetric parameters also correlate with the processes of aggregation and fusion. The interactions of non-PEGylated liposomes involve exothermic processes of higher enthalpic content than those of the PEGylated counterparts
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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7 |
|a Ligands
|2 NLM
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7 |
|a Liposomes
|2 NLM
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7 |
|a Macromolecular Substances
|2 NLM
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7 |
|a Phosphates
|2 NLM
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| 650 |
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7 |
|a Phosphatidylcholines
|2 NLM
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| 650 |
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7 |
|a Polyethylene Glycols
|2 NLM
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| 650 |
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7 |
|a 3WJQ0SDW1A
|2 NLM
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| 650 |
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7 |
|a Cholesterol
|2 NLM
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| 650 |
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7 |
|a 97C5T2UQ7J
|2 NLM
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| 650 |
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7 |
|a Guanidine
|2 NLM
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| 650 |
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7 |
|a JU58VJ6Y3B
|2 NLM
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| 700 |
1 |
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|a Tsiourvas, Dimitris
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sideratou, Zili
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Paleos, Constantinos M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Giatrellis, Sarantis
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nounesis, George
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 15 vom: 20. Juli, Seite 6165-72
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:20
|g year:2004
|g number:15
|g day:20
|g month:07
|g pages:6165-72
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