|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM149229100 |
| 003 |
DE-627 |
| 005 |
20250205161418.0 |
| 007 |
tu |
| 008 |
231223s2004 xx ||||| 00| ||chi c |
| 028 |
5 |
2 |
|a pubmed25n0498.xml
|
| 035 |
|
|
|a (DE-627)NLM149229100
|
| 035 |
|
|
|a (NLM)15238173
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a chi
|
| 100 |
1 |
|
|a Lu, Jun-Yu
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Role of bcl-2 and bax expression in apoptosis of hypoxic pulmonary artery smooth muscle cells of rats induced by Na+/H+ exchanger isoform-1 inhibition in vitro
|
| 264 |
|
1 |
|c 2004
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 08.09.2009
|
| 500 |
|
|
|a Date Revised 16.11.2017
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a OBJECTIVE: To investigate the role of bcl-2 and bax expression in apoptosis of hypoxic pulmonary artery smooth muscle cells (PASMCs) of rats induced by Na+/H+ exchanger isoform-1 (NHE-1) inhibition in vitro
|
| 520 |
|
|
|a METHODS: Cultured rat PASMCs transfected with NHE-1 ribozyme gene (PRZ cell), cultured rat PASMCs transfected only with pLXSN vector (PX cell) and non-transfected rat PASMCs (PA cell) were obtained in our previous studies. The cells were cultured under low oxygen tension (<1% O2) for 0, 2, 6, 12, 24 and 48 hours respectively. Then cell apoptosis was examined with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). Intracellular Ca2+([Ca2+]i) of cells was measured using fluorescence dye Fura-2/AM. The changes in mRNA expression of bcl-2 and bax in cells were assessed by semi quantitative reverse transcription-polymerase chain reaction (sqRT-PCR). The expression of Bcl-2 protein and Bax protein of cells was detected immunohistochemically
|
| 520 |
|
|
|a RESULTS: In contrast to normal PASMCs and cells transfected with pLXSN, the apoptosis rates in recombinant vectors transfected cells increased significantly and continuously. [Ca2+]i values were also increased in these cells, but the increase extent was remarkably lower than in the normal PASMCs (all P<0.01). The expression of bax mRNA and protein of cells transfected with recombinant vectors was increased significantly compared with cells of other two groups. Meanwhile, the expression of bcl-2 mRNA and protein of these cells were decreased significantly
|
| 520 |
|
|
|a CONCLUSION: The NHE-1 specific hammerhead ribozymes induce apoptosis of PASMCs under low oxygen tension by inhibiting the increase in intracellular Ca2+ concentration, increasing bax expression and decreasing bcl-2 expression
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Proto-Oncogene Proteins c-bcl-2
|2 NLM
|
| 650 |
|
7 |
|a RNA, Messenger
|2 NLM
|
| 650 |
|
7 |
|a Sodium-Hydrogen Exchangers
|2 NLM
|
| 650 |
|
7 |
|a bcl-2-Associated X Protein
|2 NLM
|
| 700 |
1 |
|
|a Yao, Wei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Qian, Gui-Sheng
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yang, Xiao-Jing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chen, Wei-Zhong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Shu-Ping
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 16(2004), 7 vom: 06. Juli, Seite 394-8
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
|
| 773 |
1 |
8 |
|g volume:16
|g year:2004
|g number:7
|g day:06
|g month:07
|g pages:394-8
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 16
|j 2004
|e 7
|b 06
|c 07
|h 394-8
|