Liquid-chromatography-coupled SAXS for accurate sizing of aggregating proteins

Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples o...

Description complète

Détails bibliographiques
Publié dans:Journal of synchrotron radiation. - 1994. - 11(2004), Pt 4 vom: 01. Juli, Seite 314-8
Auteur principal: Mathew, Elizabeth (Auteur)
Autres auteurs: Mirza, Ahmed, Menhart, Nick
Format: Article
Langue:English
Publié: 2004
Accès à la collection:Journal of synchrotron radiation
Sujets:Comparative Study Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Validation Study Complex Mixtures Macromolecular Substances Proteins Plasminogen plus... 9001-91-6 Cytochromes c 9007-43-6
Description
Résumé:Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results
Description:Date Completed 17.08.2004
Date Revised 10.12.2019
published: Print-Electronic
Citation Status MEDLINE
ISSN:1600-5775