Liquid-chromatography-coupled SAXS for accurate sizing of aggregating proteins
Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples o...
| Publié dans: | Journal of synchrotron radiation. - 1994. - 11(2004), Pt 4 vom: 01. Juli, Seite 314-8 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2004
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| Accès à la collection: | Journal of synchrotron radiation |
| Sujets: | Comparative Study Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Validation Study Complex Mixtures Macromolecular Substances Proteins Plasminogen plus... |
| Résumé: | Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results |
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| Description: | Date Completed 17.08.2004 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1600-5775 |