The import of phosphoenolpyruvate by plastids from developing embryos of oilseed rape, Brassica napus (L.), and its potential as a substrate for fatty acid synthesis

The plastidial phosphoenolpyruvate (PEP)/phosphate translocator (PPT) is expressed in the developing embryos of oilseed rape (Brassica napus L.). PEP can be imported by plastids isolated from embryos and used for fatty acid synthesis at rates that are sufficient to account for one-third of the rate...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 55(2004), 402 vom: 20. Juli, Seite 1455-62
1. Verfasser: Kubis, Sybille E (VerfasserIn)
Weitere Verfasser: Pike, Marilyn J, Everett, Christopher J, Hill, Lionel M, Rawsthorne, Stephen
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers Fatty Acids Phosphoenolpyruvate 73-89-2 Pyruvic Acid 8558G7RUTR
LEADER 01000naa a22002652 4500
001 NLM148948715
003 DE-627
005 20231223050704.0
007 tu
008 231223s2004 xx ||||| 00| ||eng c
028 5 2 |a pubmed24n0497.xml 
035 |a (DE-627)NLM148948715 
035 |a (NLM)15208349 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Kubis, Sybille E  |e verfasserin  |4 aut 
245 1 4 |a The import of phosphoenolpyruvate by plastids from developing embryos of oilseed rape, Brassica napus (L.), and its potential as a substrate for fatty acid synthesis 
264 1 |c 2004 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 23.09.2004 
500 |a Date Revised 21.11.2013 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a The plastidial phosphoenolpyruvate (PEP)/phosphate translocator (PPT) is expressed in the developing embryos of oilseed rape (Brassica napus L.). PEP can be imported by plastids isolated from embryos and used for fatty acid synthesis at rates that are sufficient to account for one-third of the rate of fatty acid synthesis in vivo. This provides the first experimental evidence for uptake of PEP and incorporation of carbon from it into fatty acids by plastids. PEP metabolism in isolated plastids is able to provide some of the ATP required for fatty acid synthesis. Expression of the PPT and related glucose 6-phosphate (Glc-6-P) translocator (GPT) is high in early embryo and leaf development and then declines. The marked decline in the abundance of PPT and GPT transcripts between the pre- and mid-oil accumulating stages of embryo development in B. napus does not correlate with the corresponding translocator activities, which both increase over the same period. This means that transcript abundance cannot be used to infer the activity of the translocators 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a DNA Primers  |2 NLM 
650 7 |a Fatty Acids  |2 NLM 
650 7 |a Phosphoenolpyruvate  |2 NLM 
650 7 |a 73-89-2  |2 NLM 
650 7 |a Pyruvic Acid  |2 NLM 
650 7 |a 8558G7RUTR  |2 NLM 
700 1 |a Pike, Marilyn J  |e verfasserin  |4 aut 
700 1 |a Everett, Christopher J  |e verfasserin  |4 aut 
700 1 |a Hill, Lionel M  |e verfasserin  |4 aut 
700 1 |a Rawsthorne, Stephen  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g 55(2004), 402 vom: 20. Juli, Seite 1455-62  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnns 
773 1 8 |g volume:55  |g year:2004  |g number:402  |g day:20  |g month:07  |g pages:1455-62 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 55  |j 2004  |e 402  |b 20  |c 07  |h 1455-62