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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0497.xml
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|a (DE-627)NLM148948715
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|a (NLM)15208349
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Kubis, Sybille E
|e verfasserin
|4 aut
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|a The import of phosphoenolpyruvate by plastids from developing embryos of oilseed rape, Brassica napus (L.), and its potential as a substrate for fatty acid synthesis
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 23.09.2004
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The plastidial phosphoenolpyruvate (PEP)/phosphate translocator (PPT) is expressed in the developing embryos of oilseed rape (Brassica napus L.). PEP can be imported by plastids isolated from embryos and used for fatty acid synthesis at rates that are sufficient to account for one-third of the rate of fatty acid synthesis in vivo. This provides the first experimental evidence for uptake of PEP and incorporation of carbon from it into fatty acids by plastids. PEP metabolism in isolated plastids is able to provide some of the ATP required for fatty acid synthesis. Expression of the PPT and related glucose 6-phosphate (Glc-6-P) translocator (GPT) is high in early embryo and leaf development and then declines. The marked decline in the abundance of PPT and GPT transcripts between the pre- and mid-oil accumulating stages of embryo development in B. napus does not correlate with the corresponding translocator activities, which both increase over the same period. This means that transcript abundance cannot be used to infer the activity of the translocators
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA Primers
|2 NLM
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|a Fatty Acids
|2 NLM
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|a Phosphoenolpyruvate
|2 NLM
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|a 73-89-2
|2 NLM
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|a Pyruvic Acid
|2 NLM
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|a 8558G7RUTR
|2 NLM
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1 |
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|a Pike, Marilyn J
|e verfasserin
|4 aut
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|a Everett, Christopher J
|e verfasserin
|4 aut
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|a Hill, Lionel M
|e verfasserin
|4 aut
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|a Rawsthorne, Stephen
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 402 vom: 20. Juli, Seite 1455-62
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:55
|g year:2004
|g number:402
|g day:20
|g month:07
|g pages:1455-62
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|a AR
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|d 55
|j 2004
|e 402
|b 20
|c 07
|h 1455-62
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