Biochemical characterization of a high-palmitoleic acid Helianthus annuus mutant
Copyright 2004 Elsevier SAS
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 42(2004), 5 vom: 10. Mai, Seite 373-81 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2004
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Acetates Arabidopsis Proteins Fatty Acids Fatty Acids, Monounsaturated Multienzyme Complexes palmitoleic acid 209B6YPZ4I Palmitic Acid 2V16EO95H1 plus... |
| Résumé: | Copyright 2004 Elsevier SAS In the present work we carried out analytical and biochemical studies on a new high-n-7 monounsaturated fatty acid sunflower (Helianthus annuus L.) mutant. This new line, which has been selected by classical methods of breeding and mutagenesis, shows contents of unusual acyl chains up to 20% (12% of 16:1DELTA9, 5% of 16:2delta9,12 and 6% of 18:1delta11), whereas those fatty acids are found in negligible amounts in common sunflower cultivars. This characterization involved in vivo incubations with radiolabeled acetate and measurement of the last enzymes involved in the intraplastidial de novo fatty acid synthesis: beta-ketoacyl-ACP synthase II, stearoyl-ACP desaturase (EC 1.14.19.2) and acyl-ACP thioesterases (EC 3.1.2.14). Results indicated that the high-palmitoleic acid phenotype was associated with a concerted reduction in the fatty acid synthase II activity with respect to the control lines and an increase of stearoyl-ACP desaturase activity with respect to the high-palmitate mutant line |
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| Description: | Date Completed 26.08.2004 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 0981-9428 |