Anti-endotoxin core glycolipid antibody : the preparation of immune serum of E. Coli J5
OBJECTIVE: To prepare high titer anti-endotoxin core glycolipid (J5) antibody (CGL) for the treatment of Gram-negative bacteremia and septic shock
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 16(2004), 6 vom: 07. Juni, Seite 358-60 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Bacterial Vaccines Endotoxins Glycolipids Immune Sera Immunoglobulins antilipopolysaccharide antibodies core endotoxin |
| Zusammenfassung: | OBJECTIVE: To prepare high titer anti-endotoxin core glycolipid (J5) antibody (CGL) for the treatment of Gram-negative bacteremia and septic shock METHODS: Nontoxic bacterial vaccine (50 x 10(12)U/L) against E.Coli O111:B4 mutant strain J5 was prepared. J5 bacterial vaccine was injected into rabbits through ear marginal vein (saline as control preparation), one time pre three days, totally five times. Injected doses were as following: 0.1 ml, 0.2 ml, 0.4 ml, 0.6 ml, and 0.8 ml. One week after fifth injection, blood samples from heart were collected and immune serum was isolated. Indirect clotting test was used to determine the titer of antibody and cross reaction RESULTS: Among 12 immunized rabbits, titers of antibody against E. Coli J5 were exceeding 1:1 024 in 6 rabbits, and they had cross reaction with various kinds of Gram-negative bacterial endotoxins CONCLUSION: The titer of anti-endotoxin core glycolipid (E.Coli J5) antibody prepared by us appears to be high, and it can combine with various kinds of Gram-negative bacterial endotoxins |
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| Beschreibung: | Date Completed 10.11.2004 Date Revised 08.12.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |