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20231227123409.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n1221.xml
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|a (DE-627)NLM148695116
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|a (NLM)15181112
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Kato, Chiharu
|e verfasserin
|4 aut
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|a Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 04.01.2005
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Tobacco (Nicotiana tabacum L. cv. Xanthi XHFD8) possesses four nitrite reductase (NiR) genes: nii1, nii2, nii3, and nii4. Their differential expression in leaves and roots was investigated by quantitative competitive RT-PCR using gene-specific primer pairs. These results appear to contradict existing views on the expression of these NiR genes: (i) the mRNA of each of the four NiR genes was distinguishable both in leaves and roots; (ii) nitrate treatment increased nii1 and nii3 mRNA in leaves and roots by at least 4-fold (at least 5-fold in nii2 and nii4 mRNA); and (iii) the steady-state levels of nii1 and nii3 mRNA were almost the same in leaves (6-7 x 10(5) and about 3 x 10(6) copies microg(-1) of total RNA before and after nitrate treatment, respectively) and in roots (3-4 x 10(4) and 3-6 x 10(5) copies microg(-1) of total RNA before and after nitrate treatment, respectively). Very similar relationships were obtained for the steady-state levels of nii2 and nii4 mRNA in roots (2-4 x 10(5) and 8 x 10(6) copies microg(-1) of total RNA before and after nitrate treatment, respectively), and in leaves (5-9 x 10(4) and 4 x 10(5) copies microg(-1) of total RNA before and after nitrate treatment, respectively). These results demonstrate that nii1 and nii3 transcripts are a dominating, but not exclusive, NiR mRNA in leaves, and the same is true for nii2 and nii4 transcripts in roots
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Nitrite Reductases
|2 NLM
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| 650 |
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|a EC 1.7.-
|2 NLM
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| 700 |
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|a Takahashi, Misa
|e verfasserin
|4 aut
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|a Sakamoto, Atsushi
|e verfasserin
|4 aut
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| 700 |
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|a Morikawa, Hiromichi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 403 vom: 27. Aug., Seite 1761-3
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:55
|g year:2004
|g number:403
|g day:27
|g month:08
|g pages:1761-3
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|d 55
|j 2004
|e 403
|b 27
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|h 1761-3
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