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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0495.xml
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|a (DE-627)NLM148453333
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|a (NLM)15155782
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Menckhoff, Mathias
|e verfasserin
|4 aut
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|a Transmembrane electron transport in sealed and NAD(P)H-loaded right-side-out plasma membrane vesicles isolated from maize (Zea mays L.) roots
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 03.09.2004
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Electron transport across plasma membranes has been observed in vivo in several plant species and tissues after the application of ferricyanide (hexacyanoferrate III, HCF III). In the present work, a transmembrane electron flow was demonstrated in sealed and NAD(P)H-loaded right-side-out (apoplastic-side-out) plasma membrane vesicles isolated from maize (Zea mays L.) roots. HCF III was reduced at a rate of up to 126 nmol min(-1) mg(-1) protein by NADPH-loaded vesicles, while reduction rates with NADH-loaded vesicles were several-fold lower. Coincident with the reduction of HCF III, NAD(P)H oxidation was observed inside the vesicles. The dependence of reduction on K+ indicated an electrogenic transmembrane electron flow. Application of 100 microM calcium decreased HCF III reduction up to 66%, while pre-incubation with 200 microM warfarin or diphenylene iodonium inhibited transmembrane electron transport only weakly. Fe(3+)-EDTA was not reduced significantly by NADPH-loaded plasma membrane vesicles, whereas XTT was reduced at a rate of 765 pmol min(-1) mg(-1) protein. The results suggested a major function for NADPH in transmembrane electron flow and were discussed in conjunction with in vivo experiments
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Ferricyanides
|2 NLM
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|a Onium Compounds
|2 NLM
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|a hexacyanoferrate III
|2 NLM
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|a 13408-62-3
|2 NLM
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|a NADP
|2 NLM
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|a 53-59-8
|2 NLM
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|a Warfarin
|2 NLM
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|a 5Q7ZVV76EI
|2 NLM
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|a diphenyleneiodonium
|2 NLM
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|a 6HJ411TU98
|2 NLM
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|a Edetic Acid
|2 NLM
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|a 9G34HU7RV0
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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|a Lüthje, Sabine
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 401 vom: 13. Juni, Seite 1343-9
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:55
|g year:2004
|g number:401
|g day:13
|g month:06
|g pages:1343-9
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|d 55
|j 2004
|e 401
|b 13
|c 06
|h 1343-9
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