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|a pubmed24n0492.xml
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|a (DE-627)NLM147693152
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|a (NLM)15073208
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Mikami, Koji
|e verfasserin
|4 aut
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|a Isolation of cDNAs encoding typical and novel types of phosphoinositide-specific phospholipase C from the moss Physcomitrella patens
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 03.09.2004
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|a Date Revised 15.11.2007
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|a published: Print-Electronic
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|a GENBANK: AB114834, AB117760
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|a Citation Status MEDLINE
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|a Two cDNAs encoding proteins, PpPLC1 and PpPLC2, with catalytic and C2 domains conserved in plant phosphoinositide-specific phospholipase C (PI-PLC) were isolated from Physcomitrella patens. The N domain, which has been identified in Arabidopsis PI-PLCs as an EF hand-like domain, was found in both isoforms, although that in PpPLC2 was a split type. At micromolar Ca2+ concentrations, PpPLC1 preferentially hydrolysed phosphatidylinositol-4,5-bisphosphate, while PpPLC2 showed no specificity. Furthermore, at millimolar Ca2+, phosphatidylinositol was hydrolysed by PpPLC2, but not by PpPLC1. Thus, PpPLC1 and PpPLC2 are typical and novel types of plant PI-PLC, respectively
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Phosphoinositide Phospholipase C
|2 NLM
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|a EC 3.1.4.11
|2 NLM
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|a Phosphatidylinositol Diacylglycerol-Lyase
|2 NLM
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|a EC 4.6.1.13
|2 NLM
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|a Repp, Alexander
|e verfasserin
|4 aut
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|a Graebe-Abts, Elena
|e verfasserin
|4 aut
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|a Hartmann, Elmar
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 401 vom: 25. Juni, Seite 1437-9
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:55
|g year:2004
|g number:401
|g day:25
|g month:06
|g pages:1437-9
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|a GBV_ILN_350
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|a AR
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|d 55
|j 2004
|e 401
|b 25
|c 06
|h 1437-9
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