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01000naa a22002652 4500 |
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NLM147223547 |
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DE-627 |
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20231223042857.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0491.xml
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|a (NLM)15022837
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Rodríguez-Gacio, María del Carmen
|e verfasserin
|4 aut
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|a Cloning and analysis of a cDNA encoding an endo-polygalacturonase expressed during the desiccation period of the silique-valves of turnip-tops (Brassica rapa L. cv. Rapa)
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 04.05.2004
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a During zygotic embryogenesis of turnip-tops (Brassica rapa L. cv. Rapa), the polygalacturonase activity (PG; EC 3.2.1.15), measured as a decrease in viscosity of polygalacturonic acid, reached a high when the desiccation process in the seeded silique was triggered and the valves had lost more than 70-75% of their moisture (45-50 DPA). The PG activity was not detected in any phases of developing seeds. This work also characterizes a cDNA with an open reading frame of 1303 bp and that codes for a putative PG called BrPG1. This falls into the category of clade-B, which includes PG related to shattering and abscission processes. The deduced BrPG1 sequence predicted a 434-residue-long precursor protein (46.7kDa) with a transit peptide sequence 23 amino acids long. A molecular mass of 44.3 kDa was calculated for the mature form of BrPG1, which showed high sequence similarity to PGA1 (97%) of B. napus (X98373) and ADPG1 (87%) of Arabidopsis thaliana (AJ002532). All conserved amino acids at the catalytic site of PGs belonging to clade-B were preserved on BrPG1. This BrPG1 gene was specifically expressed in the silique valves of turnip-tops and was temporally expressed at the beginning of its desiccation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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|a Polygalacturonase
|2 NLM
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|a EC 3.2.1.15
|2 NLM
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| 700 |
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|a Nicolás, Carlos
|e verfasserin
|4 aut
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|a Matilla, Angel J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 161(2004), 2 vom: 29. Feb., Seite 219-27
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:161
|g year:2004
|g number:2
|g day:29
|g month:02
|g pages:219-27
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|d 161
|j 2004
|e 2
|b 29
|c 02
|h 219-27
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