Fourier transform infrared spectroscopy as a new tool to determine rosmarinic acid in situ
A new procedure has been developed for the in situ FT-IR determination of rosmarinic acid (RA) in suspension cultures of Lavandula officinalis. The method involves sample preparation on ZnSe crystals or microplates from silicon, and measuring absorbance spectra between 4000 and 700 cm(-1). First der...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 161(2004), 2 vom: 15. Feb., Seite 151-6 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Anti-Inflammatory Agents, Non-Steroidal Cinnamates Cyclopentanes Depsides Indicators and Reagents Oxylipins jasmonic acid 6RI5N05OWW |
| Zusammenfassung: | A new procedure has been developed for the in situ FT-IR determination of rosmarinic acid (RA) in suspension cultures of Lavandula officinalis. The method involves sample preparation on ZnSe crystals or microplates from silicon, and measuring absorbance spectra between 4000 and 700 cm(-1). First derivative spectra were analysed after normalisation using partial least square (PLS) algorithm. The correlation between spectral analysis and HPLC measurements of cell extracts shows that the FT-IR procedure is suitable for qualitative and quantitative analyses of RA in cell suspension cultures |
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| Beschreibung: | Date Completed 04.05.2004 Date Revised 13.12.2023 published: Print Citation Status MEDLINE |
| ISSN: | 1618-1328 |