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DE-627 |
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20250205103049.0 |
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231223s2004 xx ||||| 00| ||chi c |
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|a pubmed25n0490.xml
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|a (DE-627)NLM147107849
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|a (NLM)15009959
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Kong, Yi-hui
|e verfasserin
|4 aut
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|a Effect of beta3-adrenoreceptors agonist on beta3-adrenoreceptors expression and myocyte apoptosis in a rat model of heart failure
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 07.10.2004
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|a Date Revised 15.11.2007
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To evaluate the effects of beta(3)-adrenoreceptor (AR) agonist (BRL-37344) on the expression of beta(3)-AR in a isoproterenol (ISO)-induced heart failure (HF) rat model and to investigate the influence on the levels of beta(3)-AR in failing heart
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|a METHODS: The rats were randomly divided into four groups: I group (control group, n=10); II group (normal with BRL group, n=10); III group (HF group, n=30); IV group (HF with BRL group, n=35).II and IV groups received BRL 0.4 nmol.kg-1.min-1 through caudal vein for 10 minutes twice a week. I and III groups received saline at the same time. The measure included hemodynamics, the expression of beta3-AR in left ventricular myocytes by the techniques of immunohistochemistry, beta3-AR proteins by western blot, expression levels of beta3-AR mRNA in myocardium by reverse transcription- polymerase chain reaction (RT-PCR) and the levels of apoptotic cells with a terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling(TUNEL) kit
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|a RESULTS: (1)Hemodynamic: The tendency of left ventricular end systolic pressure (PES), maximal rates of rise of ventricular pressure(dp/dtmax), maximal rates of decline of ventricular pressure (dp/dtmin) in II, III and IV groups were lower (P<0.01), time constant of left ventricular relaxation (Tc) and left ventricular end diastolic pressure (PED) were higher than those of the I group (all P<0.01). There was no difference between I and II group except PED (P<0.05). Compared with III group, PES, dp/dtmax, dp/dtmin in IV group were dramatically decline (P<0.05): Tc, PED were markedly increased (Tc P<0.05, PED P<0.01). (2)The levels of beta3-AR mRNA and beta3-AR proteins were higher in III and IV groups when compared with I and II groups. There was no difference between I and II group. IV group's levels were higher than III group's. (3)The apoptotic rates in III group and IV group were significantly higher than those in I and II group (all P<0.01). When compared with III group's apoptotic cell rate, IV group's was higher (P<0.01)
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|a CONCLUSION: The levels of beta(3)-AR mRNA and proteins show an increase in failing heart compared with nonfailing heart. The effect of beta(3)-AR agonist aggravate markedly cardiac function and stimulate cardiac myocytes apoptosis in failing heart. If the levels of beta(3)-AR were too high, they might contribute to the loss of cardiac function and be the foundation of the functional degradation of HF
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| 650 |
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|a Comparative Study
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| 650 |
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|a English Abstract
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Adrenergic beta-Agonists
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 650 |
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|a Receptors, Adrenergic, beta-3
|2 NLM
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| 700 |
1 |
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|a Li, Wei-min
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tian, Ying
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 16(2004), 3 vom: 01. März, Seite 142-7
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:16
|g year:2004
|g number:3
|g day:01
|g month:03
|g pages:142-7
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 16
|j 2004
|e 3
|b 01
|c 03
|h 142-7
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