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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n1253.xml
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|a (NLM)14718496
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Sousa-Majer, Maria José de
|e verfasserin
|4 aut
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|a Response to water deficit and high temperature of transgenic peas (Pisum sativum L.) containing a seed-specific alpha-amylase inhibitor and the subsequent effects on pea weevil (Bruchus pisorum L.) survival
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 13.05.2004
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|a Date Revised 09.01.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The effects of water deficit and high temperature on the production of alpha-amylase inhibitor 1 (alpha-AI-1) were studied in transgenic peas (Pisum sativum L.) that were developed to control the seed-feeding pea weevil (Bruchus pisorum L., Coleoptera: Bruchidae). Transgenic and non-transgenic plants were subjected to water-deficit and high-temperature treatments under controlled conditions in the glasshouse and growth cabinet, beginning 1 week after the first pods were formed. In the water-deficit treatments, the peas were either adequately watered (control) or water was withheld after first pod formation. The high-temperature experiments were performed in two growth cabinets, one maintained at 27/22 degrees C (control) and one at 32/27 degrees C day/night temperatures, with the vapour pressure deficit maintained at 1.3 kPa. The plants exposure to high temperatures and water deficit produced 27% and 79% fewer seeds, respectively, than the controls. In the transgenic peas the level of alpha-AI-1 as a percentage of total protein was not influenced by water stress, but was reduced on average by 36.3% (the range in two experiments was 11-50%) in the high-temperature treatment. Transgenic and non-transgenic pods of plants grown at 27/22 degrees C and 32/27 degrees C were inoculated with pea weevil eggs to evaluate whether the reduction in level of alpha-AI-1 in the transgenic pea seeds affected pea weevil development and survival. At the higher temperatures, 39% of adult pea weevil emerged, compared to 1.2% in the transgenic peas grown at the lower temperatures, indicating that high temperature reduced the protective capacity of the transgenic peas
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Enzyme Inhibitors
|2 NLM
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|a alpha-Amylases
|2 NLM
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| 650 |
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|a EC 3.2.1.1
|2 NLM
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| 700 |
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|a Turner, Neil C
|e verfasserin
|4 aut
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|a Hardie, Darryl C
|e verfasserin
|4 aut
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|a Morton, Roger L
|e verfasserin
|4 aut
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|a Lamont, Byron
|e verfasserin
|4 aut
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|a Higgins, Thomas J V
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 396 vom: 12. Feb., Seite 497-505
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:55
|g year:2004
|g number:396
|g day:12
|g month:02
|g pages:497-505
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|d 55
|j 2004
|e 396
|b 12
|c 02
|h 497-505
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