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231223s2004 xx ||||| 00| ||chi c |
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|a pubmed25n0481.xml
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|a (DE-627)NLM144163136
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|a (NLM)14706198
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Cui, Yu-fang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Radioprotection of recombinant human interleukin-3 and granulocyte-macrophage colony-stimulating factor on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy gamma-rays
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 16.06.2004
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| 500 |
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|a Date Revised 15.11.2006
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To observe the radioprotection of recombinant human interleukin-3 (rhIL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and rhIL-3 (rhIL-3+GM-CSF) on peripheral lymphocytes of rhesus monkey irradiated by 3.0 Gy gamma-rays, and attempt to provide evidence of cytokines used effectively in the therapy of acute radiation sickness
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| 520 |
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|a METHODS: Thirty rhesus monkey used in the experiment were randomly divided into six groups of rhIL-3 20 microg.kg -1.d -1, 60 microg.kg -1.d -1 GM-CSF 10 microg.kg -1.d -1 IL-3 20 microg.kg -1.d -1 +GM-CSF 10 microg.kg -1.d -1 radiation control and normal control. 21 d after whole body gamma-irradiation and subcutaneous injection of cytokines, T lymphocyte and its subsets, Bax/Bcl-2 proteins in lymphocytes were determined by immunohistochemical staining with alkaline phosphatase, and lymphocyte apoptosis was detected by TdT-mediated dUTP nick end labeling (TUNEL) technique
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|a RESULTS: (1) After irradiation the quantities of peripheral lymphocyte, T cell and its subsets obviously decreased as compared with those of normal controls. For instance, the percentages of lymphocyte, T, T H and Ts cells in radiation control group reduced to 44 percent, 42 percent, 41 percent and 57 percent of normal controls, respectively. (2)After radiation the reduction of lymphocyte, T, T H and Ts cells were evidently improved by injection of GM-CSF and GM-CSF+IL-3, The T,T H cells in GM-CSF and GM-CSF+IL-3 groups were respectively elevated by 1.57 and 1.76 fold, as well as 1.48 and 1.72 fold of radiation controls. (3) A large amount of lymphocyte apoptosis was found after radiation, GM-CSF and GM-CSF+IL-3 treatment could distinctively inhibit abundant lymphocyte apoptosis induced by acute irradiation,the apoptotic rates of lymphocytes in GM-CSF and GM-CSF+IL-3 groups reduced to 41 percent and 48 percent respectively when compared with that of radiation controls
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|a CONCLUSION: A definite dose of GM-CSF and GM-CSF+IL-3 could suppress the reduction of lymphocyte, T and T H cells and lymphocyte apoptosis induced by 3.0 Gy gamma-irradiation. It confirms that inhibition of GM-CSF and GM-CSF+IL-3 on lymphocyte reduction as well as apoptosis might be one of the major causes to alleviate radiation injury of lymphocytes and improve the immunological function
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| 650 |
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|a Comparative Study
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| 650 |
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|a English Abstract
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a BAX protein, human
|2 NLM
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| 650 |
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|a Interleukin-3
|2 NLM
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| 650 |
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7 |
|a Proto-Oncogene Proteins
|2 NLM
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| 650 |
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7 |
|a Proto-Oncogene Proteins c-bcl-2
|2 NLM
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| 650 |
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|a Recombinant Proteins
|2 NLM
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| 650 |
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|a bcl-2-Associated X Protein
|2 NLM
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| 650 |
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|a Granulocyte-Macrophage Colony-Stimulating Factor
|2 NLM
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| 650 |
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|a 83869-56-1
|2 NLM
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| 650 |
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|a Alkaline Phosphatase
|2 NLM
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| 650 |
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7 |
|a EC 3.1.3.1
|2 NLM
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| 700 |
1 |
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|a Yang, Hong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, Qing-liang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dong, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Xiao-lan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Han
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mao, Bing-zhi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, De-wen
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 16(2004), 1 vom: 05. Jan., Seite 22-5
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:16
|g year:2004
|g number:1
|g day:05
|g month:01
|g pages:22-5
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 16
|j 2004
|e 1
|b 05
|c 01
|h 22-5
|