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20231227123333.0 |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n1221.xml
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|a (DE-627)NLM143864858
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|a (NLM)14673025
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Tian, Ai-Guo
|e verfasserin
|4 aut
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| 245 |
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|a Isolation and characterization of a Pti1 homologue from soybean
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 13.05.2004
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A full-length gene GmPti1 was identified from soybean in an EST sequencing project by its homology to tomato Pti1. It encoded a protein of 366 amino acids. RT-PCR analysis showed that the GmPti1 expression was induced by salicylic acid and wounding. The deduced amino acid sequence had a Ser/Thr/Tyr kinase domain. GmPti1 protein was expressed in E. coli as an MBP fusion, purified by amylose resin and examined for its autophosphorylation ability. The phosphorylation assay in vitro showed that GmPti1 had kinase activity in the presence of Mn2+. These results demonstrated that GmPti1 represented a new Pti1-like gene, unlike the two published genes sPti1a and sPti1b, which encoding proteins had no autophosphorylation ability
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a DNA Primers
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Protein Serine-Threonine Kinases
|2 NLM
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| 650 |
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7 |
|a EC 2.7.11.1
|2 NLM
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| 700 |
1 |
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|a Luo, Guang-Zuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yong-Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jin-Song
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gai, Jun-Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Shou-Yi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 55(2004), 396 vom: 01. Feb., Seite 535-7
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:55
|g year:2004
|g number:396
|g day:01
|g month:02
|g pages:535-7
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_ILN_350
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|a AR
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|d 55
|j 2004
|e 396
|b 01
|c 02
|h 535-7
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