Production of soluble and functional engineered antibodies in Escherichia coli improved by FkpA

Production of soluble and functional engineered antibodies in Escherichia coli improved by FkpA

Overproduction of genetically engineered antibodies, such as single-chain antibodies (scAbs) in Escherichia coli often results in insoluble and inactive products known as inclusion bodies. We now report that fusion or co-expression of FkpA, the E. coli periplasmic peptidyl-prolyl-isomerase with chap...

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Veröffentlicht in:BioTechniques. - 1993. - 35(2003), 5 vom: 06. Nov., Seite 1032-8, 1041-2
1. Verfasser: Zhang, Zhong (VerfasserIn)
Weitere Verfasser: Song, Li-ping, Fang, Min, Wang, Fei, He, Dan, Zhao, Rui, Liu, Jing, Zhou, Zhi-yong, Yin, Chang-cheng, Lin, Qing, Huang, Hua-liang
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Evaluation Study Journal Article Validation Study Antibodies Escherichia coli Proteins Membrane Proteins Recombinant Fusion Proteins Immunophilins EC 5.2.1.8 mehr... Peptidylprolyl Isomerase FkpA protein, E coli EC 5.2.1.8.