Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface
Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and st...
| Veröffentlicht in: | BioTechniques. - 1993. - 35(2003), 5 vom: 06. Nov., Seite 1014-8, 1020-1 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2003
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Evaluation Study Journal Article Biocompatible Materials Membranes, Artificial Ether 0F5N573A2Y Polyethylene Glycols 3WJQ0SDW1A |
| Zusammenfassung: | Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology |
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| Beschreibung: | Date Completed 21.07.2004 Date Revised 10.12.2019 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |