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20231223030136.0 |
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231223s2003 xx ||||| 00| ||eng c |
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|a pubmed24n0477.xml
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|a (DE-627)NLM143127594
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|a (NLM)14593798
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Gómez Roig, Edurne
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Maize DNA polymerase alpha is phosphorylated by a PCNA-associated cyclin/Cdk complex
|b effect of benzyladenine
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|c 2003
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 09.02.2004
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|a Date Revised 08.12.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a The activity of maize DNA polymerases 1 and 2 (delta and alpha-type enzymes, respectively) is stimulated during germination if embryo axes are imbibed in the presence of benzyladenine. In vivo, DNA pol 2 is a phosphorotein that appears to be maximally phosphorylated previous to the S phase start time (by 12 h of germination, Coello and Vázquez-Ramos 1995a). We find that, in vitro, a PCNA-associated cyclin/kinase activity isolated from maize axes acquires an increasing capacity to phosphorylate DNA pol 2 as germination advances; moreover, the PCNA-associated kinase isolated from BA-treated maize axes germinated at 3 h phosphorylates DNA pol 2 at the same level observed in samples of axes germinated for 13 h in the absence of exogenous BA. PCNA-associated kinase activity from BA-treated axes germinated at 13 h maximal using DNA pol 2 as substrate. However, there is no modification in DNA polymerase activity as a consequence of protein phosphorylation. Results are discussed in terms of their significance for cell cycle regulation during seed germination
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Benzyl Compounds
|2 NLM
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| 650 |
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|a Cyclins
|2 NLM
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|a Cytokinins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Proliferating Cell Nuclear Antigen
|2 NLM
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| 650 |
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|a Purines
|2 NLM
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| 650 |
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|a Cyclin-Dependent Kinases
|2 NLM
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|a EC 2.7.11.22
|2 NLM
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| 650 |
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|a DNA Polymerase I
|2 NLM
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| 650 |
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|a EC 2.7.7.7
|2 NLM
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| 650 |
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|a Adenine
|2 NLM
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| 650 |
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|a JAC85A2161
|2 NLM
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| 650 |
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|a benzylaminopurine
|2 NLM
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| 650 |
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|a KXG6A989PS
|2 NLM
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| 650 |
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|a Kinetin
|2 NLM
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| 650 |
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|a P39Y9652YJ
|2 NLM
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| 700 |
1 |
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|a Vázquez-Ramos, Jorge M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 160(2003), 9 vom: 01. Sept., Seite 983-90
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:160
|g year:2003
|g number:9
|g day:01
|g month:09
|g pages:983-90
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| 912 |
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 160
|j 2003
|e 9
|b 01
|c 09
|h 983-90
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