Transformation of yeast using calcium alginate microbeads with surface-immobilized chromosomal DNA
Yeast artificial chromosomes (YACs) are useful cloning vectors that have the capacity to carry large DNA inserts. The largest barrier to using such large DNA molecules in transformation experiments has been their physical instability in solution. We developed a new method of transforming yeast using...
| Publié dans: | BioTechniques. - 1991. - 35(2003), 4 vom: 07. Okt., Seite 734-6, 738-40 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2003
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| Accès à la collection: | BioTechniques |
| Sujets: | Evaluation Study Technical Report Validation Study Journal Article Alginates Coated Materials, Biocompatible DNA, Fungal Hexuronic Acids Glucuronic Acid 8A5D83Q4RW |