Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts

Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts

The normalization and subtraction of highly expressed cDNAs from relatively large tissues before cloning dramatically enhanced the gene discovery by sequencing for the mouse full-length cDNA encyclopedia, but these methods have not been suitable for limited RNA materials. To normalize and subtract f...

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Veröffentlicht in:BioTechniques. - 1991. - 35(2003), 3 vom: 30. Sept., Seite 510-6, 518
1. Verfasser: Hirozane-Kishikawa, Tomoko (VerfasserIn)
Weitere Verfasser: Shiraki, Toshiyuki, Waki, Kazunori, Nakamura, Mari, Arakawa, Takahiro, Kawai, Jun, Fagiolini, Michela, Hensch, Takao K, Hayashizaki, Yoshihide, Carninci, Piero
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Evaluation Study Research Support, Non-U.S. Gov't Technical Report Validation Study Journal Article