Method to express and purify nm23-H2 protein from baculovirus-infected cells

Method to express and purify nm23-H2 protein from baculovirus-infected cells

High-throughput protein expression and purification are major bottlenecks in the postgenomic and proteomic era. We show here an automated method to express and purify nm23-H2, a nucleoside diphosphate kinase (NDPK), in a 96-well format, by the use of a robotic workstation, from insect Spodoptera fru...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 35(2003), 2 vom: 01. Aug., Seite 384-8, 390-1
1. Verfasser: Garzia, L (VerfasserIn)
Weitere Verfasser: André, A, Amoresano, A, D'Angelo, A, Martusciello, R, Cirulli, C, Tsurumi, T, Marino, G, Zollo, M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't NM23 Nucleoside Diphosphate Kinases Proteins Recombinant Proteins Protein Kinases EC 2.7.- Casein Kinases EC 2.7.11.1 Nucleoside-Diphosphate Kinase EC 2.7.4.6