Method to express and purify nm23-H2 protein from baculovirus-infected cells
High-throughput protein expression and purification are major bottlenecks in the postgenomic and proteomic era. We show here an automated method to express and purify nm23-H2, a nucleoside diphosphate kinase (NDPK), in a 96-well format, by the use of a robotic workstation, from insect Spodoptera fru...
| Publié dans: | BioTechniques. - 1991. - 35(2003), 2 vom: 01. Aug., Seite 384-8, 390-1 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2003
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't NM23 Nucleoside Diphosphate Kinases Proteins Recombinant Proteins Protein Kinases EC 2.7.- Casein Kinases EC 2.7.11.1 Nucleoside-Diphosphate Kinase |