A comparative study of Edwardsiella ictaluri parent (EILO) and E. ictaluri rifampicin-mutant (RE-33) isolates using lipopolysaccharides, outer membrane proteins, fatty acids, Biolog, API 20E and genomic analyses

The biological properties of Edwardsiella ictaluri RE-33 rifampicin-mutant and its parent strain EILO were analysed. RE-33 is an avirulent isolate used as a modified live vaccine against enteric septicaemia of catfish. Electrophoretic analysis of lipopolysaccharide (LPS) patterns showed high homolog...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 26(2003), 7 vom: 21. Juli, Seite 415-21
1. Verfasser: Arias, C R (VerfasserIn)
Weitere Verfasser: Shoemaker, C A, Evans, J J, Klesius, P H
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Comparative Study Journal Article Bacterial Outer Membrane Proteins Fatty Acids Lipopolysaccharides Rifampin VJT6J7R4TR
LEADER 01000naa a22002652 4500
001 NLM126957533
003 DE-627
005 20231222212942.0
007 tu
008 231222s2003 xx ||||| 00| ||eng c
028 5 2 |a pubmed24n0423.xml 
035 |a (DE-627)NLM126957533 
035 |a (NLM)12946011 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Arias, C R  |e verfasserin  |4 aut 
245 1 2 |a A comparative study of Edwardsiella ictaluri parent (EILO) and E. ictaluri rifampicin-mutant (RE-33) isolates using lipopolysaccharides, outer membrane proteins, fatty acids, Biolog, API 20E and genomic analyses 
264 1 |c 2003 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 23.12.2003 
500 |a Date Revised 30.09.2020 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a The biological properties of Edwardsiella ictaluri RE-33 rifampicin-mutant and its parent strain EILO were analysed. RE-33 is an avirulent isolate used as a modified live vaccine against enteric septicaemia of catfish. Electrophoretic analysis of lipopolysaccharide (LPS) patterns showed high homology between both isolates. Further characterization of LPS by immunoblotting revealed the main differences in LPS composition. The RE-33 isolate lacks the high molecular weight bands of LPS (HMW-LPS). Outer membrane protein analysis also showed some immunological differences between RE-33 and the EILO parent strain. Only two fingerprinting techniques, fatty acid composition analysis and Biolog phenotypic profiles, were able to discriminate between both isolates 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 7 |a Bacterial Outer Membrane Proteins  |2 NLM 
650 7 |a Fatty Acids  |2 NLM 
650 7 |a Lipopolysaccharides  |2 NLM 
650 7 |a Rifampin  |2 NLM 
650 7 |a VJT6J7R4TR  |2 NLM 
700 1 |a Shoemaker, C A  |e verfasserin  |4 aut 
700 1 |a Evans, J J  |e verfasserin  |4 aut 
700 1 |a Klesius, P H  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of fish diseases  |d 1998  |g 26(2003), 7 vom: 21. Juli, Seite 415-21  |w (DE-627)NLM098166034  |x 1365-2761  |7 nnns 
773 1 8 |g volume:26  |g year:2003  |g number:7  |g day:21  |g month:07  |g pages:415-21 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 26  |j 2003  |e 7  |b 21  |c 07  |h 415-21