The role of abscisic acid in the response of a specific vacuolar invertase to water stress in the adult maize leaf
Among the numerous molecular and physiological modifications induced by water deficit, one of the earliest events observed in maize mature leaves subjected to water deprivation was a strong enhancement of acid vacuolar invertase activity, which occurred before the classical reduction in gas exchange...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 54(2003), 390 vom: 15. Sept., Seite 2177-86 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2003
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Water 059QF0KO0R Fructose 30237-26-4 Sucrose 57-50-1 Abscisic Acid 72S9A8J5GW Glycoside Hydrolases mehr... |
| Zusammenfassung: | Among the numerous molecular and physiological modifications induced by water deficit, one of the earliest events observed in maize mature leaves subjected to water deprivation was a strong enhancement of acid vacuolar invertase activity, which occurred before the classical reduction in gas exchange due to stomatal closure. The increase in invertase activity coincided with the rapid accumulation of glucose and fructose that reached 8-fold the control leaf value. In addition, acid vacuolar invertase activity appeared to be highly correlated with xylem sap ABA concentration. In order to investigate the nature of the relationship between ABA and invertase activity, and to disconnect ABA from a likely sucrose side-effect, excised leaves were supplied with ABA or sucrose. As a consequence of ABA supply, a peak in leaf ABA appeared 4 h later which was followed by an enhancement of vacuolar invertase activity. ABA supply also produced a second maximum in leaf ABA. The transcript level of the Ivr2 gene encoding one vacuolar invertase presented the same two peaks pattern as leaf ABA, with a 2 h lag. This response was specific since the other invertase genes were not responding. Thus, ABA appeared to be a powerful enhancer of the IVR2 vacuolar invertase activity and expression. In the present conditions, the addition of sucrose had no effect on the enzyme activity |
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| Beschreibung: | Date Completed 12.11.2003 Date Revised 09.01.2024 published: Print Citation Status MEDLINE |
| ISSN: | 1460-2431 |