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DE-627 |
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20250204041038.0 |
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231222s2003 xx ||||| 00| ||chi c |
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|a pubmed25n0421.xml
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|a (DE-627)NLM126175381
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|a (NLM)12857441
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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| 100 |
1 |
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|a Wang, Guang-yi
|e verfasserin
|4 aut
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1 |
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|a Inhibition of nimodipine on production of proinflammatory by Kupffer cells in severe burned rats
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|c 2003
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 11.12.2012
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To study whether nimodipine, a dihydropyridine-type calcium channel blocker, can inhibit the production of interleukin-1beta(IL-1beta), interleukin-6(IL-6) by Kupffer cells(KC) and down-regulate its level of plasma after severe burn injury
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|a METHODS: KC of normal rats were isolated with portal vein catheter, intrahepatic digestion and density gradient centrifugation. Intracellular calcium concentration ([Ca2+]i) in individual KC after stimulated with postburn serum was assessed fluorometrically with microspectrofluorimeter. Level of IL-1beta and IL-6 in the supernatant of KC cultured with postburn serum were detected by enzyme-linked immunosorbent assay(ELISA). SD rats underwent 30% total body surface area(TBSA) full thickness burn 6 hours later, KCs was isolated and their mRNA were extracted. Level of IL-1beta mRNA and IL-6 mRNA were detected by ribonuclease protection assay(RPA). Levels of plasma IL-1beta and IL-6 were also detected. Role of nimodipine on above-mentioned effects were observed
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|a RESULTS: Compared with that of control group, levels of [Ca2+]i of KCs and IL-1beta and IL-6 supernatant in burn group increased significantly(all P<0.01). At present of 1 micromol/L nimodipine, however, the [Ca2+]i, IL-1beta, IL-6 values decreased significantly(all P<0.01). The level of plasma cytokines and KC mRNA in burn group also increased significantly. After intravenously injection with nimodipine (40 microg x kg(-1) x h(-1)), the numerical values decreased significantly(all P<0.01)
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|a CONCLUSION: Kupffer cells of rats are activated to secret IL-1beta and IL-6 after severe burn injury and this process is realized through calcium ion signal transduction channel. Nimodipine can inhibit IL-1beta and IL-6 production of KC by preventing its mRNA transcription, down-regulating its level of plasma
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|a English Abstract
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Interleukin-1beta
|2 NLM
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| 650 |
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7 |
|a Interleukin-6
|2 NLM
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| 650 |
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|a Nimodipine
|2 NLM
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| 650 |
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7 |
|a 57WA9QZ5WH
|2 NLM
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| 700 |
1 |
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|a Zhu, Shi-hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Hong-tai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ge, Sheng-de
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xia, Zhao-fan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 15(2003), 4 vom: 11. Apr., Seite 210-2
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:15
|g year:2003
|g number:4
|g day:11
|g month:04
|g pages:210-2
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 15
|j 2003
|e 4
|b 11
|c 04
|h 210-2
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