Construction of vector of his-tagged cytoplasmic fragment of human toll like receptor 4 and its expression in E.coli
OBJECTIVE: To construction of vector of his-tagged cytoplasmic fragment of human Toll like receptor 4 (hTLR4) and its expression in E.coli
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 15(2003), 3 vom: 29. März, Seite 163-6 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2003
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Membrane Glycoproteins Receptors, Cell Surface Recombinant Fusion Proteins TLR4 protein, human Toll-Like Receptor 4 Toll-Like Receptors |
| Zusammenfassung: | OBJECTIVE: To construction of vector of his-tagged cytoplasmic fragment of human Toll like receptor 4 (hTLR4) and its expression in E.coli METHODS: hTLR4 cytoplasmic cDNA codon domain was amplified by polymerase chain reaction (PCR) and cloned into pET-DsbA2.0 plasmid expressing His-DsbA fusion protein. After being identified by the assay of restrictional enzyme and sequencing, His-Dsb A fusion proteins were induced with isopropy-beta-D-thiogalactoside (IPTG) and further purified RESULTS: A fusion protein with molecular weight of 42 kd was obtained CONCLUSION: hTLR4 which was constructed and expressed successfully under nondenaturing conditions provides a tool for further studies |
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| Beschreibung: | Date Completed 23.06.2004 Date Revised 08.12.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |