Regulatory role of iron and EDTA in the shoot development from the epicotyl explants of Syzygium cuminii
Iron has proved to be an integral component of the culture medium supporting the caulogenic response of the epicotyl segments of S. cuminii. In the absence of both the iron and EDTA even the shoot buds failed to develop, while in the presence of either of these, though the shoot buds developed, thei...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 160(2003), 5 vom: 21. Mai, Seite 569-72 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2003
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Culture Media Ferric Compounds Ferrous Compounds ferrous sulfate 39R4TAN1VT ferric citrate 63G354M39Z Edetic Acid |
| Zusammenfassung: | Iron has proved to be an integral component of the culture medium supporting the caulogenic response of the epicotyl segments of S. cuminii. In the absence of both the iron and EDTA even the shoot buds failed to develop, while in the presence of either of these, though the shoot buds developed, their elongation was adversely affected. Among the three iron sources tested, ferrous sulphate proved to be the best, as the ferric chloride was not as effective as the former when used either alone or along with EDTA. Ferric citrate, on the other hand, when provided alone, elicited better response than that induced by ferrous sulphate alone. However, in combination with EDTA, the response declined significantly. The estimation of endogenous levels of iron in the explants further supported these results. The quantum of iron absorption was at a maximum during the first week of the culture and the explants, once deprived of iron during the first week, failed to catch up to the level of iron accumulated in the explants maintained continuously on complete medium, even after transfer to the complete medium. Likewise, the level of copper ions did not come up to comparable levels even if the explants were transferred to the complete medium after initial deprivation |
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| Beschreibung: | Date Completed 07.08.2003 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1618-1328 |