Extraction of total RNA from leaves of Eucalyptus and other woody and herbaceous plants using sodium isoascorbate

Extraction of total RNA from leaves of Eucalyptus and other woody and herbaceous plants using sodium isoascorbate

Rapid extraction of total RNA from Eucalyptus leaves is difficult due to the high content of polyphenolics and polysaccharides. A rapid and simple method was developed by using an extraction buffer containing sodium isoascorbate at a concentration of 500 mM. This method consisted of one or two chlor...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 34(2003), 5 vom: 14. Mai, Seite 988-90, 992-3
1. Verfasser: Suzuki, Y (VerfasserIn)
Weitere Verfasser: Hibino, T, Kawazu, T, Wada, T, Kihara, T, Koyama, H
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Research Support, Non-U.S. Gov't Technical Report Journal Article Plant Extracts RNA 63231-63-0 Ascorbic Acid PQ6CK8PD0R