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20231222204339.0 |
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231222s2003 xx ||||| 00| ||eng c |
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|a pubmed24n0416.xml
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|a (DE-627)NLM124804489
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|a (NLM)12709483
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Jones, Mark A
|e verfasserin
|4 aut
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| 245 |
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|a A simple method for obtaining cell-specific cDNA from small numbers of growing root-hair cells in Arabidopsis thaliana
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|c 2003
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 25.07.2003
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|a Date Revised 13.05.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a A simple and rapid method for cloning specific cDNAs from mRNA populations derived solely from small numbers of root-hair cells is described here. To identify genes expressed during the earliest visible stage of root-hair cell development, cell contents were aspirated from small numbers of Arabidopsis root-hair cells at or just before this stage. This material was used to make reusable solid-phase oligo-dT-primed cDNA libraries. To demonstrate that the libraries contained high quality longer cDNAs, a fragment located 2.7 kb from the 3' end of the cDNA of the single copy root-hair expressed gene RHD3 was cloned using a nested PCR strategy. This technique was also used to obtain novel gene expression information by cloning the full-length 0.85 kb cDNA of the Rop2 GTPase from this library. This approach offers a means of cloning larger cDNAs directly from small numbers of growing root-hair cells and, potentially, other epidermal cell types
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 650 |
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7 |
|a GTP-Binding Proteins
|2 NLM
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| 650 |
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|a EC 3.6.1.-
|2 NLM
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| 650 |
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|a RHD3 protein, Arabidopsis
|2 NLM
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| 650 |
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|a EC 3.6.1.-
|2 NLM
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| 650 |
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7 |
|a rho GTP-Binding Proteins
|2 NLM
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| 650 |
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7 |
|a EC 3.6.5.2
|2 NLM
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| 700 |
1 |
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|a Grierson, Claire S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 54(2003), 386 vom: 04. Mai, Seite 1373-8
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:54
|g year:2003
|g number:386
|g day:04
|g month:05
|g pages:1373-8
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 54
|j 2003
|e 386
|b 04
|c 05
|h 1373-8
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