Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR

Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR

PCR techniques have significantly improved the detection and identification of bacterial pathogens. Even so, the lack of differentiation between DNA from viable and dead cells is one of the major challenges for diagnostic DNA-based methods. Certain nucleic acid-binding dyes can selectively enter dea...

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Veröffentlicht in:BioTechniques. - 1993. - 34(2003), 4 vom: 24. Apr., Seite 804-8, 810, 812-3
1. Verfasser: Nogva, Hege Karin (VerfasserIn)
Weitere Verfasser: Drømtorp, Signe Marie, Nissen, Hilde, Rudi, Knut
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't DNA, Bacterial