Construction of cDNA libraries for highly efficient DNA sequencing from the 3' end of expressed genes
The majority of expressed sequence tag (EST) sequences available today have been derived from the 5' ends of cDNA clones. Obtaining high-quality DNA sequences from the 3' ends of oligo(dT)-primed cDNA on a large scale has been difficult because of slippage of the DNA polymerase enzyme used...
| Veröffentlicht in: | BioTechniques. - 1991. - 34(2003), 4 vom: 24. Apr., Seite 758-62, 764, 766 |
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| Format: | Aufsatz |
| Sprache: | English |
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2003
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Evaluation Study Technical Report Journal Article RNA 63231-63-0 |