The use of a genetically engineered Pseudomonas species (Shk1) as a bioluminescent reporter for heavy metal toxicity screening in wastewater treatment plant influent
Heavy metals are known to be inhibitory and toxic to the activated-sludge microbial community in biological wastewater treatment plants. Toxicity screening of aqueous mixtures of these heavy metal ions in plant influent could use both chemical and biological methods. As a biological method, luminesc...
| Veröffentlicht in: | Water environment research : a research publication of the Water Environment Federation. - 1998. - 75(2003), 1 vom: 15. Jan., Seite 21-9 |
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| Format: | Aufsatz |
| Sprache: | English |
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2003
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| Zugriff auf das übergeordnete Werk: | Water environment research : a research publication of the Water Environment Federation |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Metals, Heavy Sewage |
| Zusammenfassung: | Heavy metals are known to be inhibitory and toxic to the activated-sludge microbial community in biological wastewater treatment plants. Toxicity screening of aqueous mixtures of these heavy metal ions in plant influent could use both chemical and biological methods. As a biological method, luminescent bacterial bioreporters offer the advantages of a simple test procedure and rapid response. Current biologically based methods for screening aqueous streams for toxicity are labor-intensive, inaccurate, or difficult to use in continuous monitoring applications. In the present study, a system was developed that is simple and easily automated. This system is based on the bacterium Shk1, a genetically engineered bioluminescent Pseudomonad whose parent strain was originally isolated from activated sludge. Compared with other bioluminescence-based systems (specifically, the Microtox assay), the system of the present study more accurately reflects the effects of the toxicity of common metal ions on activated-sludge respirometry without being overly sensitive to typical constituents of wastewater. The use of Shk1 as a bioluminescent reporter for heavy metal toxicity testing for the application of wastewater treatment influent toxicity screening is presented in this study |
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| Beschreibung: | Date Completed 25.06.2003 Date Revised 22.09.2019 published: Print Citation Status MEDLINE |
| ISSN: | 1554-7531 |