Myocyte production of nitric oxide in response to AChR-reactive antibodies in two inbred rat strains may influence disease outcome in experimental myasthenia gravis

In an attempt to identify mechanisms that explain the difference in susceptibility of two rat strains to the induction of experimental autoimmune myasthenia gravis (EAMG), acetylcholine receptor (AChR)-reactive antibodies were tested for their ability to up-regulate levels of inducible nitric oxide...

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Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 106(2003), 2 vom: 29. Feb., Seite 116-26
1. Verfasser: Garcia, Yvonne R (VerfasserIn)
Weitere Verfasser: Pothitakis, Jennifer C, Krolick, Keith A
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S. Autoantibodies Enzyme Inhibitors Muscle Proteins N(6)-(1-iminoethyl)lysine RNA, Messenger Receptors, Cholinergic Nitric Oxide mehr... 31C4KY9ESH Nitric Oxide Synthase EC 1.14.13.39 Nitric Oxide Synthase Type II Nos2 protein, rat Lysine K3Z4F929H6
Beschreibung
Zusammenfassung:In an attempt to identify mechanisms that explain the difference in susceptibility of two rat strains to the induction of experimental autoimmune myasthenia gravis (EAMG), acetylcholine receptor (AChR)-reactive antibodies were tested for their ability to up-regulate levels of inducible nitric oxide synthase (iNOS) in skeletal muscles of disease-sensitive Lewis rats and disease-resistant Wistar Furth (WF) rats. Initially, the WF muscle cell line, WE1, appeared to be more sensitive to antibody-stimulated iNOS induction and NO production than did the Lewis muscle cell line, LE1. Next, AChR-reactive antibody induced widespread iNOS production in skeletal muscles of WF rats, while iNOS production in muscles of Lewis rats was much less pronounced. Finally, inhibition of iNOS activity by administration of a specific iNOS inhibitor resulted in increased susceptibility to the induction of impaired muscle function in EAMG-resistant WF rats. It is speculated that nitric oxide production plays a protective immunomodulating role in WF rats
Beschreibung:Date Completed 01.05.2003
Date Revised 07.11.2019
published: Print
Citation Status MEDLINE
ISSN:1521-7035