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20250203213728.0 |
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231222s2003 xx ||||| 00| ||eng c |
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|a pubmed25n0414.xml
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|a (DE-627)NLM124283535
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|a (NLM)12654869
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wu, Zhencai
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Isolation and characterization of a cDNA encoding a lipid transfer protein expressed in 'Valencia' orange during abscission
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| 264 |
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|c 2003
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 07.07.2003
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|a Date Revised 13.05.2019
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|a published: Print
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|a GENBANK: AF369931
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|a Citation Status MEDLINE
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|a The genetics and expression of a lipid transfer protein (LTP) gene was examined during abscission of mature fruit of 'Valencia' orange. A cDNA encoding an LTP, CsLTP, was isolated from a cDNA subtraction library constructed from mature fruit abscission zones 48 h after application of a mature fruit-specific abscission agent, 5-chloro-3-methyl-4-nitro-pyrazole (CMN-pyrazole). A full-length cDNA clone of 652 nucleotides was isolated using 5' and 3' RACE followed by cDNA library screening and PCR amplification. The cDNA clone encoded a protein of 155 amino acid residues with a molecular mass and isoelectric point of 9.18 kDa and 9.12, respectively. A partial genomic clone of 505 nucleotides containing one intron of 101 base pairs was amplified from leaf genomic DNA. Southern blot hybridization demonstrated that at least two closely related CsLTP genes are present in 'Valencia' orange. Temporal expression patterns in mature fruit abscission zones were examined by northern hybridization. Increased expression of CsLTP mRNA was detected in RNA of mature fruit abscission zones 6, 24, 48, and 72 h after application of a non-specific abscission agent, ethephon. Low expression of CsLTP transcripts was observed after treatment of CMN-pyrazole until 24 h after application. After this time, expression markedly increased. The results suggest that CsLTP has a role in the abscission process, possibly by assisting transport of cutin monomers to the fracture plane of the abscission zone or through its anti-microbial activity by reducing the potential of microbial attack
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Antigens, Plant
|2 NLM
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| 650 |
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7 |
|a Carrier Proteins
|2 NLM
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| 650 |
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7 |
|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a Organophosphorus Compounds
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Pyrazoles
|2 NLM
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| 650 |
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7 |
|a lipid transfer proteins, plant
|2 NLM
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| 650 |
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7 |
|a 5-chloro-3-methyl-4-nitro-1H-pyrazole
|2 NLM
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| 650 |
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7 |
|a 6814-58-0
|2 NLM
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| 650 |
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7 |
|a ethephon
|2 NLM
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| 650 |
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7 |
|a XU5R5VQ87S
|2 NLM
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| 700 |
1 |
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|a Burns, Jacqueline K
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 54(2003), 385 vom: 01. Apr., Seite 1183-91
|w (DE-627)NLM098182706
|x 0022-0957
|7 nnns
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| 773 |
1 |
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|g volume:54
|g year:2003
|g number:385
|g day:01
|g month:04
|g pages:1183-91
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 54
|j 2003
|e 385
|b 01
|c 04
|h 1183-91
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